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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
09/03/2020 |
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Data da última atualização: |
20/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SIMAS, P. V. M; BASSETTO, C. C.; GIGLIOTI, R.; OKINO, C. H.; OLIVEIRA, H. N. de; OLIVEIRA, M. C. de S. |
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Afiliação: |
Paulo Vitor Marques Simas, UNESP; César Cristiano Bassetto, UNESP; Rodrigo Giglioti, Instituto de Zootecnia; CINTIA HIROMI OKINO, CPPSE; Henrique Nunes de Oliveira, UNESP; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
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Título: |
Use of molecular markers can help to understand the genetic diversity of Babesia bovis. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Infection, Genetics and Evolution, v. 79, 2020. 104161. |
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Páginas: |
9 |
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ISSN: |
1567-1348 |
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DOI: |
https://doi.org/10.1016/j.meegid.2019.104161 |
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Idioma: |
Inglês |
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Conteúdo: |
Cattle babesiosis is a tick-borne disease responsible for significant losses for the livestock industries in tropical areas of the world. These piroplasms are under constant control of the host immune system, which lead to a strong selective pressure for arising more virulent or attenuated phenotypes. Aiming to better understand the most critical genetic modifications in Babesia bovis genome, related to virulence, an in silico analysis was performed using DNA sequences from GenBank. Fourteen genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80 (or Bb-1), 18S rRNA, acs-1, ama-1, ?-tub, cp-2, p0, rap-1a) related to parasite infection and immunogenicity and ITS region were selected for alignment and comparison of several isolates of Babesia bovis from different geographic regions around the world. Among the 15 genes selected for the study of diversity, only 7 genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80) and the ITS region presented sufficient genetic variation for the studies of phylogeny. Despite this genetic diversity observed into groups, there was not sufficient information available to associate molecular markers with virulence of isolates. However, some genetic groups no were correlated with geographic region what could indicate some typical evolutionary characteristics in the relation between parasite-host. Further studies using these genes in herds presenting diverse clinical conditions are required. The better understanding of evolutionary mechanisms of the parasite may contribute to improve prophylactic and therapeutic measures. In this way, we suggest that genes used in our study are potential markers of virulence and attenuation and have to be analyzed with the use of sequences from animals that present clinical signs of babesiosis and asymptomatic carriers. MenosCattle babesiosis is a tick-borne disease responsible for significant losses for the livestock industries in tropical areas of the world. These piroplasms are under constant control of the host immune system, which lead to a strong selective pressure for arising more virulent or attenuated phenotypes. Aiming to better understand the most critical genetic modifications in Babesia bovis genome, related to virulence, an in silico analysis was performed using DNA sequences from GenBank. Fourteen genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80 (or Bb-1), 18S rRNA, acs-1, ama-1, ?-tub, cp-2, p0, rap-1a) related to parasite infection and immunogenicity and ITS region were selected for alignment and comparison of several isolates of Babesia bovis from different geographic regions around the world. Among the 15 genes selected for the study of diversity, only 7 genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80) and the ITS region presented sufficient genetic variation for the studies of phylogeny. Despite this genetic diversity observed into groups, there was not sufficient information available to associate molecular markers with virulence of isolates. However, some genetic groups no were correlated with geographic region what could indicate some typical evolutionary characteristics in the relation between parasite-host. Further studies using these genes in herds presenting diverse clinical conditions are required. The better understanding of evolutionary mechanisms of th... Mostrar Tudo |
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Palavras-Chave: |
Attenuation; Genetic diversity; Molecular markers. |
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Thesagro: |
Babesia Bovis. |
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Thesaurus Nal: |
Virulence. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/211610/1/Use-of-molecular-markers-can-help-to-understand-the-2020-Infection-Genetics.pdf
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Marc: |
LEADER 02625naa a2200277 a 4500
001 2121033
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008 2020 bl uuuu u00u1 u #d
022 $a1567-1348
024 7 $ahttps://doi.org/10.1016/j.meegid.2019.104161$2DOI
100 1 $aSIMAS, P. V. M
245 $aUse of molecular markers can help to understand the genetic diversity of Babesia bovis.$h[electronic resource]
260 $c2020
300 $a9
520 $aCattle babesiosis is a tick-borne disease responsible for significant losses for the livestock industries in tropical areas of the world. These piroplasms are under constant control of the host immune system, which lead to a strong selective pressure for arising more virulent or attenuated phenotypes. Aiming to better understand the most critical genetic modifications in Babesia bovis genome, related to virulence, an in silico analysis was performed using DNA sequences from GenBank. Fourteen genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80 (or Bb-1), 18S rRNA, acs-1, ama-1, ?-tub, cp-2, p0, rap-1a) related to parasite infection and immunogenicity and ITS region were selected for alignment and comparison of several isolates of Babesia bovis from different geographic regions around the world. Among the 15 genes selected for the study of diversity, only 7 genes (sbp-2, sbp-4, trap, msa-1, msa-2b, msa-2c, Bv80) and the ITS region presented sufficient genetic variation for the studies of phylogeny. Despite this genetic diversity observed into groups, there was not sufficient information available to associate molecular markers with virulence of isolates. However, some genetic groups no were correlated with geographic region what could indicate some typical evolutionary characteristics in the relation between parasite-host. Further studies using these genes in herds presenting diverse clinical conditions are required. The better understanding of evolutionary mechanisms of the parasite may contribute to improve prophylactic and therapeutic measures. In this way, we suggest that genes used in our study are potential markers of virulence and attenuation and have to be analyzed with the use of sequences from animals that present clinical signs of babesiosis and asymptomatic carriers.
650 $aVirulence
650 $aBabesia Bovis
653 $aAttenuation
653 $aGenetic diversity
653 $aMolecular markers
700 1 $aBASSETTO, C. C.
700 1 $aGIGLIOTI, R.
700 1 $aOKINO, C. H.
700 1 $aOLIVEIRA, H. N. de
700 1 $aOLIVEIRA, M. C. de S.
773 $tInfection, Genetics and Evolution$gv. 79, 2020. 104161.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Acre. |
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Data corrente: |
20/10/2016 |
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Data da última atualização: |
16/11/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
COSTA, J. B. da; DICK, D. P.; LIMA, M. O.; LARA, L. Z.; PERALBA, M. do C.; COSTA, F. de S. |
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Afiliação: |
Janaina Berne da Costa, Universidade Federal do Rio Grande do Sul; Deborah Pinheiro Dick, Universidade Federal do Rio Grande do Sul; Maurifran Oliveira Lima, Universidade Federal do Rio Grande do Sul; Larissa Zacher Lara, Universidade Federal do Rio Grande do Sul; Maria do Carmo Peralba, Universidade Federal do Rio Grande do Sul; FALBERNI DE SOUZA COSTA, CPAF-Acre. |
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Título: |
Biomarker analysis in soils of the Amazon rainforest after vegetation fire. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: SETAC EUROPE ANNUAL MEETING, 26., 2016, Nantes. Proceedings... Brussels: SETAC, 2016. |
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Páginas: |
2 p. |
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Idioma: |
Inglês |
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Conteúdo: |
The objective of this study was to determine the origin of organic matter incorporated in Amazon forest soils subjected to vegetation fire by analyzing the aliphatic biomarkers (n-alkanes) present in lipid extracts of soil samples. |
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Palavras-Chave: |
Acre; Amazônia Ocidental; Biomarcador alifático; Extrato lipídico; Floresta primária; Rio Branco (AC). |
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Thesagro: |
Marcador Molecular; Matéria Orgânica; Queimada; Solo. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/149031/1/26154.pdf
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Marc: |
LEADER 01106nam a2200301 a 4500
001 2055093
005 2023-11-16
008 2016 bl uuuu u00u1 u #d
100 1 $aCOSTA, J. B. da
245 $aBiomarker analysis in soils of the Amazon rainforest after vegetation fire.$h[electronic resource]
260 $aIn: SETAC EUROPE ANNUAL MEETING, 26., 2016, Nantes. Proceedings... Brussels: SETAC$c2016
300 $a2 p.
520 $aThe objective of this study was to determine the origin of organic matter incorporated in Amazon forest soils subjected to vegetation fire by analyzing the aliphatic biomarkers (n-alkanes) present in lipid extracts of soil samples.
650 $aMarcador Molecular
650 $aMatéria Orgânica
650 $aQueimada
650 $aSolo
653 $aAcre
653 $aAmazônia Ocidental
653 $aBiomarcador alifático
653 $aExtrato lipídico
653 $aFloresta primária
653 $aRio Branco (AC)
700 1 $aDICK, D. P.
700 1 $aLIMA, M. O.
700 1 $aLARA, L. Z.
700 1 $aPERALBA, M. do C.
700 1 $aCOSTA, F. de S.
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Registro original: |
Embrapa Acre (CPAF-AC) |
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